原著論文 ／ 英文総説 ／ 和文総説

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●原著論文

Dhimas Agung Kurniawan, Sylvia Leo, Mutsumi Inamatsu, Sohei Funaoka, Taichi Aihara, Takeshi Sakura, Hiroshi Arakawa, Yukio Kato, Tomoaki Matsugi, Katsuhiro Esashika, Nobuaki Shiraki, Shoen Kume, Hiroshi Kimura, Masaki Nishikawa, Yasuyuki Sakai, "Gut-Liver Microphysiological Systems Revealed Potential Crosstalk Mechanism Modulating Drug Metabolism", PNAS NEXUS 3(2): pgae070. doi: 10.1093/pnasnexus/pgae070. eCollection 2024 Feb.

Hiyoshi N, Enomoto T, Uefune F, Kato Y, Wu Y, Araki K, Sakano D, Shiraki N, Kume S. A specific plasma amino acid profile in the Insulin2Q104del Kuma mice at the diabetic state and reversal from hyperglycemia.Biochem. Biophys. Res. Commun. 679,58-65, 2023. doi.org/10.1016/j.bbrc.2023.08.064

Helena GA, Watanabe T, Kato Y, Shiraki N, Kume S. Activation of cAMP(EPAC2) signaling pathway promotes hepatocyte attachment. Scientific Reports  13, 12352, 2023. DOI: 10.1038/s41598-023-39712-3

Tokuma H, Sakano D, Tanabe, Tanizawa Y, Shiraki N, Kume S. Selective proteasome degradation of the C-terminally-truncated human WFS1 mutants in the pancreatic beta cells. FEBS Open Bio. 13, 1391-1540, 2023. Doi/10.1002/2211-5463.13674

Leo S, Kato Y, Wu Y, Yokota M, Koike M, Yui S, Tsuchiya K, Shiraki N, Kume S. The effect of Vitamin D3 and Valproic Acid on the maturation of human induced pluripotent stem cell-derived enterocyte-like cells. Stem Cells 41(8):775-791, 2023. Doi/10.1093/stmcls/sxad042.

Sim Z.E, Enomoto T, Shiraki N, Kume S. Protocol to generate human PSC-derived pancreatic beta-cells through methionine and zinc deprivation. STAR Protocols. 102183, 2023. doi: 10.1016/j.xpro.2023.102183.

Ali M, Kato Y, Shiraki N, Kume S. Generation of induced pluripotent stem cell-derived beta-cells in blood amino acids-like medium. Biology Open 12, bio059581, 2023. Doi:10.1242/bio.059581

Ozawa H., Kambe A, Hibi K, Murakami S, Oikawa A, Handa T, Fujiki K, Nakato R, Shirahige K, Kimura H, Shiraki N, Kume S. Transient methionine deprivation triggers histone modification and potentiated differentiation of induced pluripotent stem cells. Stem Cells 41, 271-286. 2023. PMID: 36472570, DOI: 10.1093/stmcls/sxac082

Sim Z.E, Enomoto T, Shiraki N, Furuta N, Kashio S, Kambe T. Tsuyama T. Arakawa A, Ozawa H. Yokoyama M, Miura M, Kume S. “Methionine metabolism regulates pluripotent stem cell pluripotency and differentiation through zinc mobilization.” Cell Reports 40, 111120, 2022. Doi.org/10.1016/j.celrep.2022.111120

Uefune F, Aonishi T, Kitaguchi T, Takahashi H, Seino S, Sakano D, Kume S, “Dopamine negatively regulates insulin secretion through D1-D2 receptor heteromer.” Diabetes 74, 1946-1961, 2022.  https://doi.org/10.2337/db21-0644 online published

Asawa Y, Nishida K, Domae K, Kawai K, Ban HS, Kitazaki A, Asami H, Kohno J, Okada S, Tokuma H, Sakano D, Kume S, Tanaka M, Nakamura H. “Carborane as an Alternative Efficient Hydrophobic Tag for Protein Degradation.” BioConjugate Chem. 32(11):2377-2385, 2021. Nov 17 PMID: 34699716 DOI: 10.1021/acs.bioconjchem.1c0043 Epub 2021 Oct 26.

Taki K, Takagi H, Hirose T, Sun R., Yaginuma H, Mizoguchi A, Kobayashi T, Sugiyama M, Tsunekawa T, Onoue T, Hagiwara D, Ito Y, Iwama S, Suga H, Banno R, Sakano D, Kume S, Arima H, Dietary sodium chloride attenuates increased β-cell mass to cause glucose intolerance in mice under a high-fat diet, PLosOne, 16(3): e0248065, 2021.

Shinohara M, Arakawa H, Oda Y, Shiraki N, Sugiura S, Nishiuchi T, Satoh T, Iino K, Leo S,, Kato Y, Araya K, Kawanishi T, Nakatsuji T, Mitsuta M, Inamura K, Goto T, Shinha K, Nihei W, Komori K, Nishikawa M, Kume S, Kato Y, Kanamori T, Sakai Y, Kimura H. Coculture of hiPS-derived intestinal cells and enhanced human hepatocyte functions in a pneumatic pressure-driven two-organ microphysiological system. Sci. Reports 11, 5437, 2021. Doi.org/10.1038/s41598-021-84861-y

Yoshida S, Honjo T, Iino K, Ishibe R, Leo S, Shimada T, Watanabe T, Ishikawa M, Maeda K, Kusuhara H, Shiraki N, Kume S. Generation of human induced pluripotent stem cell-derived functional enterocyte-like cells for pharmacokinetic studies, Stem Cell Reports 16, 295-308, 2021. Doi: 10.1016/j.stemcr.2020.12.017

Sakano D, Uefune F, Tokuma H, Sonoda Y, Matsuura K, Takeda N, Nakagata N, Kume K, Shiraki N, Kume S.　VMAT2 safeguards beta-cells against dopamine cytotoxicity under high-fat diet induced stress. Diabetes 69, 1-15, 2020.online ahead of print. Aug 21, 2020.https://dx.doi.org/10.2337/db20-0207

Sakano D, Inoue A, Enomoto T, Imasaka M, Okada S, Yokota M, Koike M, Araki K, Kume S.  Insulin2 Q104del (Kuma) Mutant Mice Develop Diabetes with Dominant Inheritance. Sci Reports 2020. doi: 10.1038/s41598-020-68987-z.

Yoshida S, Hirota M, Kawachi T, Kume S, Takahashi K. Generation of intestinal organoids derived from human pluripotent stem cells for drug testing. Sci Reports 10(1), 5989, 2020. doi.org/10.1038/s41598-020-63151-z. 2020. PMID: 32249832.

Nakai S, Shibata I, Shitamichi T, Yamaguchi H, Takagi N, Inoue T, Nakagawa T, Kiyokawa J, Wakabayashi S, Miyoshi T, Higashi E, Ishida S, Shiraki N, Kume S, Collagen vitrigel promotes hepatocytic differentiation of induced pluripotent stem cells into functional hepatocyte-like cells. Biology Open 8, bio042192, 2019. doi: 10.1242/bio.042192.

Kaitsuka T, Kojima R, Kawabe M, Noguchi H, Shiraki N, Kume S and Tomizawa K. A Culture Substratum with Net-like Polyamide Fibers Promotes the Differentiation of Mouse and Human Pluripotent Stem Cells to Insulin-Producing Cells. Biomedical Materials 14(4):045019, 2019; doi: 10.1088/1748-605X/ab261c.

Matsuura K, Ito K, Shiraki N, Kume S, Hagiwara N, Shimizu T. iPS cell elimination in a cell sheet by methionine-free and 42 °C condition for tumor prevention. Tissue Eng Part C Methods. 24, 605-615, 2018. doi: 10.1089/ten.TEC.2018.0228.

Tsukamoto K, Cnop M, Mori D, Kume S, Anazawa T, Doi M, Chikazawa K, Matsumaru N. Future perspectives for the treatment of diabetes: importance of a regulatory framework. Therapeutic Innovation & Regulatory Science 53(4):535-541, 2019. doi: 10.1177/2168479018795854.

Shoji M, Minato H, Ogaki S, Seki M, Suzuki Y, Kume S and Kuzuhara T, Different murine-derived feeder cells alter the definitive endoderm differentiation of human induced pluripotent stem cells. PLosONE, 13(7): e0201239, 2018.　 https://doi.org/10.1371/journal.pone.0201239

Liu KC, Leuckx G, Sakano D, Seymour PA, Mattssona CL, Rautioa L, Verdonck Yannick, Serup P, Kume S, Heimberg H, Andersson O. Inhibition of Cdk5 Promotes β-cell differentiation from ductal progenitors. Diabetes 67, 58-70, 2018.

Hasegawa T, Jun Tomita J, Hashimoto R, Ueno T, Kume S, Kume K. Sweetness induces sleep through gustatory signaling independent of nutritional value in a starved fruit fly. Sci. Rep. 2017 Oct 30;7(1):14355. doi: 10.1038/s41598-017-14608-1.

Kaitsuka T, Kobayashi K, Otsuka W, Kubo T, Hakim F, Wei FY, Shiraki N, Kume S and Tomizawa K. Erythropoietin facilitates definitive endodermal differentiation of mouse embryonic stem cells via activation of ERK signaling. Am. J. of Physiology - Cell Physiology, 312(5), 2017. doi: 10.1152/ ajpcell.00071.2016.

Koga T, Shiraki N, Yano S, Suico MA, Morino-Koga S, Sato T, Shuto T, Kume S, Kai H. Mild electrical stimulation with heat shock guides differentiation of embryonic stem cells into Pdx1-expressing cells within the definitive endoderm. BMC Biotechnology 17(1), 14, 2017. Doi:10.1186/s12896-017-0331-z

Omori H, Ogaki S, Sakano D, Sato M, Umeda K, , Nakagata N, Kume S. Expression of novel pancreatic endocrine cells gene, C2cd4c changes during pancreatic development. FEBS Lett 590, 2584-93, 2016.

Sakano D., Choi S, Kataoka M, Shiraki N, Uesugi M, Kume K, Kume S. Dopamine D2 receptor-mediated regulation of beta cell mass.Stem Cell Report 7, 95-109, 2016.

Ogaki S, Omori H, Morooka M, Shiraki N, Ishida S, Kume S. Late stage definitive endodermal differentiation can be defined by Daf1 expression. BMC Dev Biol.16:19, 2016.

Nakahara Y, Muto A, Sakuma T, Yamamoto T, Kume S, and Kikuchi Y. Temporal effects of Notch signaling and potential cooperation with multiple downstream effectors on adenohypophysis cell specification in zebrafish. Genes Cells 21(5):492-504,2015.

Arakawa A, Shiraki N, Tsuyama T, Kume S, Iwahata D, Yamada N. Quantification of intracellular Zn, Fe, Cu within both iPS cells and differentiated cells using HPLC coupled to ICP-MS. (*inductively coupled plasma mass spectrometer). J. Anal Bioanal Tech 7, Issue 1, 1000295, 2016.

Ogaki S, Morooka M, Otera K and Kume S. A cost effective system for differentiation of intestinal epithelialium from human induced pluripotent stem cells. Sci Rep 5: 17297, 2015.

Nakashima R, Morooka M, Shiraki N, Sakano D, Ogaki S, Kume K, Kume S, Neural cells play an inhibitory role in pancreatic differentiation of pluripotent stem cells. Genes Cells 20, 1028-1045, 2015.

Tomita J, Ueno T, Mitsuyoshi M, Kume S and Kume K. The NMDA receptor promotes sleep in the fruit fly, Drosophila melanogaster. PLoS ONE 10(5):e0128101,2015.

Suzuki K, Numada T, Suzuki H, Raga D D, Ipulan L A, Yokoyama C, Matsushita S, Hamada M, Nakagata N, Nishinakamura R, Kume S, Takahashi S and Yamada G. Sexually dimorphic expression of MafB regulates masculinization of the embryonic urethral formation. Proc. Nat. Acad. Sci. 111, 16407-16412, 2014.

Shahjalal HM, Shiraki N, Sakano D, Kikawa H, Ogaki S, Baba H, Kume K., Kume S. Generation of insulin-producing beta-like cells from human iPS cells in a defined and completely Xeno-free culture system.J. Mol. Cell Biol. 6, 394-408, 2014.　

Nakashima R, Yano T, Ogawa J, Tanaka N, Toda N, Yoshida M, Takano R, Inoue M, Honda T, Kume S and Matsumoto K. Potentiation of insulin secretion and improvement of glucose intolerance by combining a novel G protein-coupled receptor 40 agonist DS-1558 with glucagon-like peptide-1 receptor agonists. Eur. J. Pharmacol. 737, 194-201, 2014.

Shiraki N., Shiraki Y., Tsuyama T., Obata F, Miura M, Nagae G, Aburatani H., Kume K, Endo F, Kume S. Methionine metabolism regulates maintenance and differentiation of human pluripotent stem cells. Cell Metab. 19, 780-794, 2014.

Kikawa K, Sakano D, Shiraki N, Kume K, Endo F, Kume S. The beneficial effect of insulin treatment on the outcome of islet transplantation in Akita mice. PLoS ONE 9(4): e95451, 2014.

Hakim F, Kaitsuka T, Mohd RJ, Wei FY, Shiraki N, Akagi T, Yokota T, Kume S, Tomizawa K. High Oxygen Condition Facilitates the Differentiation of Mouse and Human Pluripotent Stem Cells into Pancreatic Progenitors and Insulin-Producing Cells. J. Biol. Chem. 289(14):9623-38, 2014.

Isono K, Jono H, Ohya Y, Shiraki N, Yamazoe T, Sugasaki A, Era T, Fusaki N, Tasaki M, Ueda M, Shinriki S, Inomata Y, Kume S, and Ando Y. Generation of familial amyloidotic polyneuropathy-specific induced pluripotent stem cells．Stem Cell Res. 12, 574-583, 2014.

Sakano D, Shiraki N, Kikawa K, Yamazoe T, Kataoka M, Umeda K, Araki K, Mao D, Matsumoto S, Nakagata N, Andersson O, Stainier D, Endo F, Kume K，Uesugi M and Kume S. VMAT2 identified as a regulator of late-stage beta cell differentiation. Nat. Chem. Biol. 10, 141-148, 2014.

Kaitsuka T, Noguchi H, Shiraki N, Kubo T, Wei FY, Hakim H, Kume S, and Tomizawa K. Generation of functional insulin-producing cells from mouse embryonic stem cells through 804G cell-derived extracellular matrix and protein transduction of transcription factors. Stem Cells Transl. Med. 3, 114-27, 2014.

Yamazoe T, Shiraki N, Toyoda M, Kiyokawa N, Okita H, Miyagawa Y, Akutsu H, Umezawa A, Sasaki Y, Kume K and Shoen Kume. A synthetic nanofibrillar matrix promotes in vitro hepatic differentiation of embryonic stem cells and induced pluripotent stem cells. J. Cell Sci. 126, 5391-5399, 2013.

Kawabata M, Ueno T, Tomita J, Kawatani J, Tomoda A, Kume S, Kume K. Temporal organizations of rest defined by actigraphy data in healthy and childhood chronic fatigue syndrome children. BMC Psychiatry 13:281, 2013.

Iwashita H*, Shiraki N, Sakano D, Shiga M, Sasamoto K, Kume K., and Kume S. Secreted Cerberus1 as a marker for quantification of definitive endoderm differentiation of the pluripotent stem cells. PLoS ONE 8(5): e64291, 2013.

Ogaki S, Shiraki N, Kume K, Kume S. Wnt and Notch signals guide embryonic stem cell differentiation into the intestinal lineages. Stem Cells 31, 1086-1096, 2013.

Umeda K, Suzuki K, Yamazoe T, Shiraki N, Higuchi Y, Tokieda K, Kume K, Mitani K., Kume S. Albumin gene targeting in human embryonic stem cells and induced pluripotent stem cells with helper-dependent adenoviral vector to monitor hepatic differentiation. Stem Cell Res. 10, 179-194, 2013.

Kataoka M, Kawamuro Y, Shiraki N, Miki R, Sakano D, Yoshida T, Yasukawa T, Kume K, Kume S. Recovery from diabetes in neonatal mice after a low-dose streptozotocin treatment. Biochem. Biophys. Res. Commun 430, 1103-1108, 2013.

Ueno T, Tomita J, Tanimoto H, Ito K, Kume S, Kume K. Identification of a dopamine pathway that regulates sleep and arousal in Drosophila. Nat Neurosci 15, 1516-1523, 2012.

Ueno T, Masuda N, Kume S, and Kume K.Dopamine modulates the rest period length without perturbation of its power law distribution in Drosophila melanogaster PLoS One 7(2):e32007, 2012.

Ueno T, Tomita J, Kume S, Kume K.Dopamine modulates metabolic rate and temperature sensitivity in Drosophila melanogaster PLoS ONE 7(2):e31513, 2012.

Miki R, Yoshida T, Murata K, Oki S, Kume K and Kume S. Fate maps of ventral and dorsal pancreatic progenitor cells in early somite stage mouse embryos. Mech. Dev. 128, 597-809, 2012.

Yamazaki M, Tomita J, Takahama K, Ueno T, Mitsuyoshi M, Sakamoto E, Kume S and Kume K. High calorie diet augments age-associated sleep impairment in Drosophila. Biochem. Biophys. Res. Commun 417, 812-816, 2012.

Takahama K, Tomita J, Ueno T, Yamazaki M, Kume S and Kume K.  Pan-neuronal knockdown of the c-Jun N-terminal Kinase (JNK) results in reduction in sleep and longevity in Drosophila. Biochem. Biophys. Res. Commun. 417, 807-811, 2012.

Isagawa T, Nagae G, Shiraki N, Fujita T, Sato N, Ishikawa S, Kume S, Aburatani H., DNA methylation profiling of embryonic stem cell differentiation into the three germ layers. PLoS ONE 6(10): e26052. 2011.

Tomita J, Mitsuyoshi M, Ueno T, Aso Y, Tanimoto H, Nakai Y, Aigaki T, Kume S and Kume K. Pan-neuronal knockdown of calcineurin reduces sleep in the fruit fly, Drosophila melanogaster.  J. Neurosci. 31, 13137-13146, 2011.

Shiraki N, Yamazoe T, Qin Z, Ohgomori K, Mochitate K, Kume K, Kume S. Efficient differentiation of embryonic stem cells into hepatic cells in vitro using a feeder-free basement membrane substratum. PLoS ONE 6(8), e24228, 2011.

Akiyama, M., Shiraishi E., Sakugawa T, Hosseini, SHR. Akiyama H., Shiraki N. and Kume S. Influence of 60 ns Pulsed electric fields on embryonic stem cells. IEEE transactions on DEI (Dielectrics and Electrical Insulation). Vol. 18, Issue 4, pp. 1119-1123, 2011.

Nagae G, Isagawa T, Shiraki N, Suemori H, Kume S, Aburatani H. Tissue-specific demethylation in CpG-poor promoters during cellular differentiation. Hum. Mol. Genet. 20(14), 2710－2721, 2011.

Katsumoto K and Kume S. Endoderm and mesoderm reciprocal signaling mediated by CXCL12 and CXCR4 regulates the migration of angioblasts and establishes the pancreatic fate. Development 138, 1947-1955, 2011

Ogaki S, Harada S, Shiraki N, Kume K, and Kume S. An expression profile analysis of ES cell-derived definitive endodermal cells and Pdx1-expressing cells. BMC Dev. Biol. 11:13, 2011.

Matsuo A, Yoshida T, Yasukawa T, Miki R., Kazuhiko K. and Kume S. Epiplakin1 is expressed in the cholangiocyte lineage cells in normal liver and adult progenitor cells in injured liver. MOD-Gene Expr Patterns. 11, 255-262, 2011.

Shiraki S, Higuchi Y and Kume S. Guiding ES cell differentiation into the definitive endoderm lineages. Inflamm. Regen. 30, 109-114, 2010.

Higuchi Y, Shiraki N., Yamane K, Qin Z, Mochitate K, Araki K., Senokuchi K, Yamagata K, Hara M, Kume K, and Kume S*. Synthesized basement membranes direct the differentiation of mouse embryonic stem cells into pancreatic lineages. J. Cell Sci. 123, 2733-2742, 2010.

Shiraki N, Harada S, Ogaki S, Kume K. and Kume S. Identification of DAF1/CD55, a novel definitive endoderm marker. Cell Struct. Funct. 35, 73-80, 2010

Yoshida T, Guo X, Namekata K, Mitamura Y, Kume S. and Harada  T. Expression of Epiplakin 1 in the developing and adult mouse retina. Jpn J Ophthalmol. 54, 85-88. 2010.

Katsumoto K, Shiraki N, Miki R and Kume S. Embryonic and adult stem cell systems in mammals: Ontology and regulation” in ‘Comparative aspects of Stem cells. Dev. Growth Differ. 52, 115-129, 2010.

Matsuura K*, Katsumoto K*, Fukuda K, Kume K and Kume S. Conserved origin of the ventral pancreas in chicken. Mech. Dev.126, 817-827, 2009.

Katsumoto K, Fukuda K, Kimura W, Shimamura K, Yasugi S and Kume S.  Origin of pancreatic precursors in the chick embryo and the mechanism of endoderm regionalization. Mech. Dev. 126, 539-551, 2009.

Yoshida T, Murata K, Shiraki N, Kume K and Kume S. Analysis of gene expressions of embryonic stem -derived Pdx1-expressing cells: Implications of genes involved in pancreas differentiation. Dev. Growth Differ. 51, 463-472, 2009.

Shiraki N, Higuchi Y, Harada S, Umeda K, Isagawa T, Aburatani H, Kume K and Kume S. Differentiation and characterization of embryonic stem cells into three germ layers. Biochem. Biophys. Res. Comm. 381, 694-9, 2009.

Shiraki N, Umeda K, Sakashita N, Takeya M, Kume K and Kume S. Differentiation of mouse and human ES cells into hepatic lineages. Genes Cells 13, 731-746, 2008.

Yoshida T, Shiraki N, Baba H, Goto M, Fujiwara S, Kume K and Kume S. Expression patterns of epiplakin1 in pancreas, pancreatic cancer and regenerating pancreas. Genes Cells 13, 667-678, 2008.

Shiraki N, Yoshida T, Araki K, Umezawa A, Higuchi Y, Goto H, Kume K, and Kume S. Guided differentiation of embryonic stem cells into Pdx1-expressing regional-specific definitive endoderm. Stem Cells 26, 874-885, 2008.

Sugiki T, Uyama T, Toyoda1 M, Morioka H, Kume S, Miyado K, Matsumoto K, Saito H., Tsumaki N, Takahashi Y, Toyama Y, and Umezawa A. Hyaline cartilage formation and enchondral ossification modeled with KUM5 and OP9 chondroblasts. J. Cell. Biochem. 100: 1240-1254, 2007.

Kume K, Kume S, Park SK, Hirsh J and Jackson FR. Dopamine is a regulator of arousal in the fruit fly. J. Neurosci. 25, 7377-84, 2005.

Ohmuraya M, Hirota M, Araki M, Mizushima N, Matsui M, Mizumoto T, Haruna K, Kume S, Takeya M, Ogawa M, Araki K, Yamamura K-I. Autophagic cell death of pancreatic acinar cells in serine protease inhibitor Kazal type 3-deficient mice. Gastroenterology12: 696-705, 2005.

Shiraki N, Lai C-J, Hishikari Y and Kume S. TGF-β signaling potentiates differentiation of embryonic stem cells to Pdx-1 expressing endodermal cells. Genes Cells 10, 503-16, 2005.

Kodama S, Toyonaga T, Kondo T, Matsumoto K, Tsuruzoe K, Kawashima J, Goto H, Kume K, Kume S, Sakakida M and Araki E. Enhanced expression of PDX-1 and Ngn3 by exendin-4 during beta cell regeneration in STZ-treated mice. Biochem. Biophys. Res. Comm. 327, 1170-1178, 2005.

Saneyoshi T, Kume S and Mikoshiba K. Calcium/calmodulin- dependent protein kinase I in Xenopus laevis. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 134: 499-507, 2003.

Rajagopal J, Anderson W J, Kume S, Martinez OI and Melton DA. Insulin staining of ES cell progeny from insulin uptake. Science299, 363, 2003.

Natsume T, Taoka M, Manki H, Kume S, Isobe T and Mikoshiba K. Rapid analysis of protein interactions: On-chip micropurification of recombinant protein expressed in Esherichia coli. Proteomics 2, 1247-1253, 2002.

Saneyoshi T, Kume S, Amasaki Y, and Mikoshiba K. The Wnt/calcium pathway activates NF-AT and promotes ventral cell fate in Xenopus embryos. Nature 417, 295-299, 2002.

Saneyoshi T§, Kume,S＊.§, Natsulme T and Mikoshiba K. (§Equal contribution, Molecular cloning and expression profile of Xenopus calcineurin A subunit. Biochem. Biophys. Acta. 1499, 164-170, 2000.

Kume S, Inoue T and Mikoshiba K. Gas family G proteins activate IP3-Ca2+ signaling via Gbg and tranduce ventralizing signals in Xenopus Dev. Biol. 226, 88-103, 2000.

Kume S, Saneyoshi T and Mikoshiba K. Desensitization of IP3-induced Ca2+ release by overexpression of a constitutively active Gqa protein converts ventral to dorsal fate in Xenopus early embryos. Dev. Growth Differ. 42, 327-335, 2000.

Kume S. Role of the inositol 1,4,5-trisphosphate receptor in early embryonic development. Cell. Mol. Life Sci. 56, 296-304, 1999.

Kume S, Muto A, Inoue T, Suga K, Okano H and Mikoshiba, K. Role of Inositol 1, 4, 5-trisphosphate receptor in ventral signaling in Xenopus embryos. Science 278, 1940-1943, 1997.

Kume S, Muto A, Okano H and Mikoshiba K. Developmental expression of the inositol 1,4,5-trisphosphate receptor and localization of inositol 1,4,5-trisphosphate during early embryogenesis in Xenopus laevis. Mech. Dev. 66, 157-168, 1997.

Umemori H, Inoue T, Kume S, Sekiyama N, Nagao M, Itoh H, Nakanishi S, Mikoshiba K and Yamamoto T. Activation of the G protein Gq/11 through tyrosine phosphorylation of the a subunit.  Science 276, 1878-1881, 1997. 

Kume S, Yamamoto A, Inoue T, Muto A, Okano H and Mikoshiba K.  Developmental expression of the inositol 1,4,5-trisphosphate receptor and structural changes in the endoplasmic reticulum during oogenesis and meiotic maturation of Xenopus laevis. Dev. Biol. 182, 228-239, 1997.

Muto A, Kume S, Inoue T, Okano H and Mikoshiba K. Calcium waves along the cleavage furrows in cleavage-stage Xenopus embryos and its inhibition by heparin. J. Cell Biol.135, 181-190, 1996.

Honda Z, Takano T, Hirose N, Suzuki T, Muto A, Kume S, Mikoshiba K, Itoh K and Shimizu T. Gq pathway desensitizes chemotactic receptor-induced calcium signaling via inositol trisphosphate receptor down-regulation. J. Biol. Chem. 270, 4840-4844, 1995.

Mikoshiba K, Furuichi T, Miyawaki A, Yoshikawa S, Nakade S, Michikawa T, Nakagawa T, Okano H, Kume S, Muto A, Aruga J, Yamada N, Hamanaka Y, Fujino I, Kobayashi M. Structure and Function of Inositol 1,4,5-Trisphosphate Receptor. Annual. New York Acad. Sci. 707, 178-197, 1993.

Kume S, Muto A, Aruga J, Nakagawa T, Michikawa T, Furuichi T, Nakade S, Okano H and Mikoshiba K. The Xenopus InsP3 receptor: structure, function and localization in oocytes and eggs, Cell 73, 555-570, 1993.

Yamamoto T, Masuko K, Takada S, Kume S and Obinata M. A balance between self-renewal and commitment in the murine erythroleukemia cells with the transferred c-myc gene; an in vitro stochastic model. Cell Differ. Dev.28, 129-134, 1989.

Kaneko-Ishino T, Kume S, Sasaki H, Obinata M and Oishi M.  Effect of c-myc gene expression on early inducible reactions required for erythroid differentiation in vitro. Mol. Cell. Biol. 8, 5545-5548, 1988.

Kume S, Takada S and Obinata M. Probability that the commitment of murine erythroleukemia cell differentiation is determined by the c-myc level, J. Mol. Biol. 202, 779-786, 1988.

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●英文総説

Shimaya Y, Shiraki N, Kume S. Promoting pancreatic β cell proliferation: a powerful key for realizing regenerative therapy for diabetes. J Diabetes Investig. DOI:10.1111/jdi.13967

Sim Z.E, Shiraki　N, Kume S. Recent progress in pancreatic islet cell therapy. Inflammation and Regeneration 41(1):1, 2021. doi: 10.1186/s41232-020-00152-5.

Shiraki S, Kume S. Detailed analysis at a single-cell level of cells undergoing pancreatic differentiation. J Diabetes Investig. 11(1):20-21, 2020. doi: 10.1111/jdi.13140. (commentary)

Kume S. Heterogeneity of beta-cells. J Diabetes Investig. doi 10.1111/jdi.12608

Tsuyama T, Shiraki N, Kume S. “Definitive endoderm differentiation of human embryonic stem cells combined with selective elimination of undifferentiated cells by methionine deprivation”, Methods Mol Biol. 2015 [Epub ahead of print]

Sakano D, Shiraki N, Kume S. Pancreatic differentiation from murine ES cells. Methods Mol Biol. 2015 [Epub ahead of print]

Umeda K, Shiraki N, Kume S, Hepatic differentiation from human iPS cells using M15 cells, Methods Mol Biol. 2014 Nov 23. [Epub ahead of print] DOI 10.1007/7651_2014_146

Yamazoe T, Shiraki N, Kume S, Hepatic differentiation from murine and human iPS cells using nanofiber scaffolds, Methods Mol Biol. 2014 Nov 20. Epub. DOI 10.1007/7651_2014_138

Shiraki N, Ogaki S, Kume S*. Profiling of embryonic stem cell differentiation. Rev Diabet Stud. 11(1):102-14, 2014.

Ogaki S and Kume S. Intestinal differentiation of mouse ES cells. Bio-protocol. www.bio-protocol.org　http://www.bio-protocol.org/e1011

Ogaki S and Kume S. Intestinal differentiation of human ES cells. Bio-protocol. www.bio-protocol.org　http://www.bio-protocol.org/e1010

Katsumoto K and Kume S. The role of CXCL12-CXCR4 signaling pathway in pancreatic development. Theranostics 3, 11-17, 2013.

Katsumoto K, Miki R, Umeda K, Shiraki N, Kume S. Chapter 9. ENDODERM” (p225-252) in “Stem Cell Biology: Basic Concepts to Frontiers Students Edition”, ISBN-10: 1466291079.

Shiraki N, Miki R, Katsumoto K, Kume S. The potential of ES cells and tissue stem cells in the regenerative medicine of type I diabetes. ‘Stem Cell Therapy, 71-85, 2011. Chapter 5. ISBN: 978-81-7895-502-5

Higuchi Y, Shiraki N, Kume S. In vitro models of pancreatic differentiation using ES or iPS cells. Congenital Anomalies, 51, 21–25, 2011.

Kume S. Stem cell-based approaches for regenerative medicine. Dev. Growth Differ. 47, 393-402, 2005.

Kume, S. The molecular basis and prospects in pancreatic development. Dev. Growth Differ. 47, 367-74, 2005.

Kume S. Role of the inositol 1,4,5-trisphosphate receptor in early embryonic development. Cell. Mol. Life Sci. 56, 296-304, 1999.

Kume, S. Xenopus embryos and ES cells as tools for studies of developmental biology. Neurochem. Res. 36, no.7, 1280-1285, 2011.

Kume S, Okano H and Mikoshiba K. IP3 receptor mediates ventralizing signals in Xenopus embryos. Neurochem. Res. 24 (1) 125, 1999.

Mikoshiba K, Furuichi T, Miyawaki A, Michikawa T, Yamamoto-Hino M, Hirota J, Takei K, Inoue T, Kume S, Matsumoto M and Muto A. Role of inositol 1,4,5-trisphosphate (IP3) receptor/Ca2+ signaling in cell function. Chalenges for neuroscience in the 21st century Taniguchi symposia on brain sciences No. 22. 121-146, 1999.

Kume S, Muto A, Aruga J, Okano H and Mikoshiba K. Role of InsP3 receptor in Ca2+ signaling and egg activation. RIKEN Review 6, 19-20, 1994.

Mikoshiba K, Furuichi T, Miyawaki A, Yoshikawa S, Nakade S, Michikawa T, Nakagawa T, Okano H, Kume S, Muto A, Aruga J, Yamada N, Hamanaka Y, Fujino I, Kobayashi M. Structure and Function of Inositol 1,4,5-Trisphosphate Receptor. Annual. New York Acad. Sci. 707, 178-197, 1993.

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●和文総説

白木伸明　粂昭苑「メチオニンと亜鉛は多能性幹細胞の多能性および分化を制御する」生化学 95巻5号pp. 1-5 (2023).

粂昭苑「膵臓β細胞の再生医療について」さかえ　10月号pp. 25-30 (2023).

坂野大介　粂昭苑「新しい時代の1型糖尿病治療―再生医療の現状もふまえて」糖尿病プラクティス 39 (6): 625-629, 2022．

坂野大介　粂　昭苑「膵臓内分泌細胞の発生と分化における1細胞レベルにおける理解」糖尿病学2020、p26-33.

白木伸明　粂昭苑「臓器チップに要求される細胞組織ソースと細胞共用培地」「細胞」（藤田聡史　産総研・先端フォトニックスバイオセンシングOIL）【特集　創薬におけるMPS技術の開発】(2): 174-177, 2020.

坂野大介　粂　昭苑「膵β細胞再生療法の最前線」『糖尿病・内分泌代謝科』第50巻第2号 特集／膵島研究UPDATE　50(2)：1-6, 2020．

粂　昭苑　最前線　ファルマシア「多能性幹細胞を用いた内胚葉由来の組織の作製」ファルマシア Vol 55, No6, 527-520, 2019.

粂　昭苑　最近のトピックス「創薬応用へ向けた幹細胞由来ヒト腸細胞研究の現状」薬剤学78-4, 170-174, 2018. https://doi.org/10.14843/jpstj.78.170

白木伸明　粂　昭苑「ES/iPS 細胞から膵β細胞への分化制御と糖尿病再生医療の現状と展開」特集・代謝関連疾患と合併症の再生医療の最前線「内分泌・糖尿病・代謝内科」第47巻第1号7-11, 2018

粂　昭苑　新膵臓病学　第2章膵の発生　(南江堂) p11-20（2017年）下瀬川徹　編集

白木伸明　粂昭苑「iPS細胞と肝胆膵疾患―膵臓・肝臓への分化―」GI Research第24巻2号30-36, 2016年

坂野大介　粂昭苑「膵β細胞の再生」Bio Clinic 31(3), 28-32, 2016 北隆館（川口義弥 編集）

白木伸明　粂昭苑「アミノ酸代謝によるヒト多能性幹細胞の維持・分化制御」 (岡野栄之 編集)Medical Science Digest (MSD) Vol 42(2)2-3, 2016.

坂野大介　粂昭苑「糖尿病における再生医療の現状と展望」『医療情報誌animus No.84 糖尿病最新トピックス』No 84, 35-38, 2015

大垣総一郎　粂昭苑「ES/iPS細胞からの内胚葉組織形成」生化学　87巻5号573-581,2015年（10月号）

粂昭苑「iPS細胞技術を用いた膵β細胞の分化誘導研究」『シンポジウム6：糖尿病合併症を克服する新戦略』糖尿病合併症 29, 2015（株式会社 メディカル・ジャーナル社）

白木伸明　粂昭苑「ES/iPS細胞から膵臓β細胞への分化誘導」糖尿病学 2015,１-9. 診断と治療社

白木伸明　粂昭苑「iPS細胞の内胚葉系への誘導」『再生医療―新たな医療を求めて』日本臨床73(5), 107-114，2015。

坂野大介　粂昭苑「膵発生」『特集／肝胆膵分野の再生医療・人工臓器』肝胆膵　第70巻3号, 433-439, 2015年3月号

坂野大介　粂昭苑　「Basic & Clinical Topics」基礎①　Pegliuca FW, et al, Generation of functional human pancreatic beta cells in vitro. Cell 2014; 159; 428-439. 「Diabetes Update」Vol 4, no2, 12-13, 2015

白木伸明　粂昭苑「幹細胞維持と分化におけるメチオニン代謝」『がん幹細胞―新しい医療を求めて―』（III．基礎研究動向）　日本臨床73巻5号　765-772, 2015年

坂野大介　粂昭苑「３．ケミカルバイオロジーを応用した機能的β細胞作製」『糖尿病領域における再生医療の現状と展望』　医学出版「月刊糖尿病」(川口義弥　企画編集)Vol7, No.3, 25-35

坂野大介　粂昭苑　第2章　膵臓再生の新技術「β細胞分化ーモノアミントランスポーターによる分化制御」『膵島の再生医療』36-40, 2015

白木伸明　粂昭苑「糖尿病の再生医療」『糖尿病のすべてー糖尿病対策と将来の根治・予防』(企画：門脇孝)医学のあゆみ　252, 647-653, 2015.

粂昭苑　ニホンベーリンガーインゲルハイム株式会社提供『The 1st Diabetes Research Innovation Symposium 2014』 『糖尿病成因解明の最前線』カンファレンス報告　座長のまとめ「ヒト多能性幹細胞からの立体的膵組織作製」　実験医学33，139-141、2015

白木伸明「ES/iPS細胞分化制御におけるアミノ酸代謝」アミノ酸研究8（1）5-10．

坂野大介　粂昭苑　多能性幹細胞から膵β細胞への分化を制御するシグナルislet Equality Vol.3 No.3、2014．

白木伸明　粂昭苑『ES/iP細胞を用いた内胚葉細胞（膵、肝、小腸）への分化誘導』iPS細胞研究最前線ー疾患モデルから臓器再生まで　医歯薬出版社株式会社「医学のあゆみ」251, 1153-1159, 2014

坂野大介　粂昭苑「VMAT2とβ細胞分化」(科学評論社)内分泌・糖尿病・代謝内科39, 153-159, 2014.

坂野大介　粂昭苑「ES細胞を用いた発生分化の研究と再生医学への応用」『特集　器官の発生と再生の基礎』公益財団法人金原一郎医学医療振興財団(医学書院) 生体の科学65. 197-202, 2014.

粂　昭苑「糖尿病の再生医療の実現へ向けて　膵β細胞の分化を制御するシグナル」“Signals guiding differentiation of pluripotent stem cells into pancreatic beta cells”Nihon Yakurigaku Zasshi,日薬理誌　(Folia Pharmacol. Jpn.) 144, 8-12, 2014.

坂野大介　粂昭苑「多能性幹細胞から膵β細胞への分化誘導」『特集：臨床医でもわかる糖尿病基礎研究』ホルモンと臨床　医学の世界社　60, 951-958,2014．

白木伸明　粂昭苑「メチオニンの代謝はヒトのES細胞およびiPS細胞の未分化維持および分化を制御している」First Author’s http://first.lifesciencedb.jp/archives/8655

坂野大介　粂昭苑　「小胞型モノアミン輸送体VMAT2は膵臓β細胞の分化を制御する」First Author’s　http://first.lifesciencedb.jp/archives/8151

坂野大介　粂昭苑「膵β細胞・膵島の再生研究」“Recent studies in regeneration of pancreatic β cells.”最新医学 69巻1号36-41，2014．

坂野大介　粂昭苑「iPS細胞を用いた膵β細胞研究の最前線」Diabetes Journal Vol 42,no 1, 2014.

山添太士、白木伸明)、佐々木裕)、粂昭苑　「幹細胞研究におけるES細胞,iPS細胞の利用　（肝細胞研究会　研究交流）http://hepato.umin.jp/kouryu/kouryu27.html

坂野大介　粂昭苑「幹細胞からβ細胞を誘導するシグナル」“膵島のバイオロジーの新たな展開“　Medical Review Co. Ltd Diabetes Frontier vol 24, 544-549, 2013．

白木伸明　粂昭苑「ES/iPS細胞から内胚葉組織への分化誘導方法の開発」“The establishment of endoderm differentiation methods of ES/iPS cells”Dojin review　No. 149/2013, 1－6.

白木伸明　粂昭苑【開発編】第2章「iPS細胞・ES細胞の開発応用」『再生医療・細胞培養の開発と市場』シーエムシー出版 ISBNコード： 978-4-7813-0814-2　2013年

山添太士、白木伸明、佐々木裕、粂　昭苑　肝臓の再生医療　｢日本医師会雑誌｣ vol 142, no.4, 791-795,2013．

勝本恵一　粂昭苑「膵臓の初期発生機構の解明と再生医療への応用」特集『膵β細胞の発生生物学と再生医療の応用』内分泌・糖尿病・代謝内科（科学評論社）Vol 36 No.3, 169-175, 2013.

大垣総一郎　粂昭苑「消化器幹細胞：膵上皮」『Surgery Frontier』 (Medical Review Co. Ltd) Vol.20 No.1, 70-72, 2013

坂野大介・粂昭苑『発生・分化・再生と代謝関連臓器」「多能性幹細胞から膵β細胞への分化」『内分泌・糖尿病・代謝内科』科学評論社 35, 101-106, 2012.

白木伸明・粂昭苑　最前線『躍進する幹細胞研究』「ES/iPS細胞から膵臓への分化誘導」、ファルマシア、日本薬学会（東京），48(9), 852-856, 2012.

勝本恵一、粂昭苑「膵発生：膵臓前駆細胞の起源と領域化」『胆と膵の再生医学の最前線』胆と膵　Vol.32.No 11, 1201-1206, 2011.

白木伸明　粂昭苑　第1編　再生医療に必要な"材料"とは　第1章　細胞（ES細胞・iPS細胞）『ものづくりからみる再生医療-細胞研究・創薬・治療-』シーエムシー出版 10-16, 2011.

坂野大介、白木 伸明、粂昭苑 「iPS細胞による糖尿病医療研究のこれから」-The view of stem cell therapy for type I diabetes-　糖尿病 54, 268-270, 2011.

樋口裕一郎、白木伸明、粂昭苑「膵β細胞分化誘導研究が発生学に与えるインパクト」特集号『代謝制御の鍵を握る膵β細胞』実験医学28、1364-1367, 2010.

山添太士・白木伸明、粂昭苑「ES細胞由来分化肝細胞の創出」『ヒト幹細胞による薬物代謝・トランスポート・副作用予測ーiPS・ES細胞・間葉系幹細胞を用いた新たな創薬スクリーニング　医学の歩み232(2) 105-109,2010.

粂昭苑『１型糖尿病お役立ちマニュアル』第３章 1型糖尿病を治すー1型糖尿病根治への道｢iPS細胞による膵β細胞の誘導と分化｣日本IDDMネットワーク刊行 Part4, 68-75, 2010.

白木伸明、樋口裕一郎、粂昭苑「各種幹細胞を用いた消化器官の再生医療」『再生医療の現状と進歩～ES細胞、iPS細胞と体性幹細胞の臨床への応用～』 血液フロンテイア19, no.11,1673-1678, 2009.

坂野大介　白木伸明　粂昭苑「iPS細胞を用いた膵β細胞分化誘導の研究」『医学と医療の最前線』(社団法人日本内科学会雑誌）98, no.12, 3148-3153, 2009.

小椋光　中尾光善　粂昭苑　西中村隆一「発生医学の共同研究拠点」の船出ー日本で唯一「発生医学」を標榜する文部科学大臣認定拠点ー再生医療 8,401－406, 2009．

三木梨可　粂昭苑「肝臓、膵臓の再生を支える幹細胞システムはどうのようになっているのか？」『特集：幹細胞を用いた消化器再生医療の展望』先端医学社　分子消化器病 6,no.4,332-337,2009.

梅田香穂子・白木伸明・粂昭苑「iPS細胞からの膵臓細胞誘導と臨床応用」「臨床検査」　53巻no.10,シリーズ最新医学講座/II「iPS細胞」9, 1203-1207,2009.

白木伸明、粂昭苑「ES細胞から膵細胞への分化」　特集『肝胆膵領域における幹細胞研究の最前線』アークメーデイア(株）「肝胆膵」誌59巻4号,611-617,2009.

梅田香穂子　粂昭苑「再生医療の現状と将来」化学と教育57,no.10, 446-449,2009.

樋口裕一郎・粂昭苑「iPS細胞を用いた膵臓細胞の作製と、その移植医療への応用」特集『移植医療におけるiPS細胞研究のインパクト』谷口英樹編集　日本移植学会『移植』第44巻3号p236-240,2009.

樋口裕一郎、白木伸明、粂昭苑「iPS細胞を用いた膵臓細胞の作製技術と臨床応用への展望」『iPS細胞の産業的応用技術』シーエムシー出版　225-228, 2009.

粂昭苑「一枚の写真館：ES細胞から膵細胞への分化誘導」秀潤社「細胞工学」28, 1,2009

粂昭苑「ES/iPS細胞を用いた膵臓β細胞の作製」クリシアン　575，94-98, 2009.

白木伸明、粂昭苑「ES細胞からの内胚葉系細胞の誘導」監修　黒木登志夫　編集　許南浩　中村幸夫 『培養細胞実験ハンドブック改訂第２版』羊土社（ 東京）, 297 - 301, 2008

白木伸明・粂昭苑「ES細胞を用いた膵臓の再生医学」（河盛隆造編集）新時代の糖尿病学（3）-病因･診断・治療研究の進歩ー第2版 日本臨床66, 482-487, 2008.

勝本恵一・粂昭苑「膵及び膵外におけるPdx1の発現調節と機能」『特集/膵β細胞発生分化にかかわる転写因子の発現調節と機能』（門脇　孝編集）内分泌･糖尿病科26(2), 115-120,2008.

粂昭苑「ＥＳ細胞から体性幹細胞を作る」共立出版刊行　シリーズ 「幹細胞技術の現状と展望」蛋白核酸酵素52(7):796-800, 2007. Kume S. Tanpakushitsu Kakusan Koso. 2007 Jun;52(7):796-800. Review. Japanese.

白木伸明・粂昭苑　「ES細胞から膵β細胞への誘導」（山中伸弥他編集）分子細胞治療6, 117-134, 2007.

粂昭苑「ヒトＥＳ細胞を用いた肝胆膵の発生分化と再生医学研究」医歯薬出版刊行〈中辻憲夫他編集）『ヒトＥＳ細胞研究のネクストステージ』医学のあゆみ220, 153-157, 2007.

粂昭苑・谷口英樹「消化器官における幹細胞研究の動向」Kume S, Taniguchi H. [Prospects for stem cell biology of the pancreas]　Tanpakushitsu Kakusan Koso. 2007 Feb;52(2):139-44. Review. Japanese.共立出版刊行（菊池裕編集）『内胚葉分化の分子メカニズム』特集号　蛋白核酸酵素 52, 139-144, 2007

勝本恵一・粂　昭苑「膵臓の分化誘導シグナルと糖尿病モデル動物」メデイカルドウ刊行「シグナル伝達病を知る－その分子機序解明から新たな治療戦略まで－」(菅村和夫他編集）遺伝子医学MOOK 6号、260-265, 2006.

粂昭苑・白木伸明 Kume S, Shiraki N. Pancreatic development and stem cell-based regenerative medicine. Rinsho Byori. 2006 Apr;54(4):386-92. Review. Japanese. 臨床病理 54:386-392, 2006.

白木伸明・粂昭苑　「ES細胞から膵β細胞への誘導」「ここまで進んだ幹細胞研究と再生医療2006」（田賀哲也他編集）実験医学24 no.2, 182-187, 2006.

吉田 哲,粂昭苑「膵臓β細胞の発生・再生」羊土社刊行「キーワードで理解する発生・再生イラストマップ」（上野直人他編集）116-122, 2005.

粂昭苑「消化管領域の再生の細胞源-膵臓の発生と再生を中心に-」Frontiers in Gastroenterology 8(4): 328-334, 2003.

粂昭苑「膵の分化誘導と細胞系譜」実験医学21: 1037-1042, 2003.

粂昭苑「幹細胞を用いた膵臓再生への展望」Drug delivery System 18-2: 104-110, 2003.

粂昭苑「膵臓−発生分化の様式と誘導シグナル」Molecular Medicine 40: 176-184, 2003.

粂昭苑「膵臓の幹細胞を用いた再生医療研究」分子細胞治療1: 51-55, 2002.

粂昭苑「膵臓の発生・分化と膵共通前駆細胞」実験医学19, 106-111,2001.

粂昭苑, 御子柴克彦「IP3-Ca2+ シグナル伝達系と初期発生」蛋白質、核酸、酵素 43 ,1834-1840 (1998)

粂昭苑　「IP3-Ca2+ シグナルの背腹軸形成における役割」実験医学 16 ,1202-1204 (1998)

粂昭苑　「IP3-Ca2+ シグナル伝達系とアフリカツメガエルの背腹軸」細胞工学 17 ,408-410 (1998)

粂昭苑, 御子柴克彦 「カルシウムのはたらきでカエルの背を腹に」遺伝 52 ,6-7 (1998)